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Ethics, reproducibility and methodological pitfalls Frequent pitfalls in cognitive peptide research No vehicle control in some old Russian studies: mandatory in any modern publication Non-randomisation : assigning animals to groups by order of arrival introduces systematic bias (weight, oestral cycle, cage stress) Non-blinded experimenter : behavioural tests are highly sensitive to expectations

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A knockdown study and a small-molecule mouse study are exactly the steps that should precede a human trial, and they are the reason a compound gets taken seriously enough to be studied further

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Regular monitoring identifies when adjustments become necessary